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KMID : 1189420150280020001
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2015 Volume.28 No. 2 p.1 ~ p.12
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Study on the Anti-aging Activity of Gentianae sino-ornata Ethanol Extract
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Choi Hyung-Wook
Li Shun-Hua
Kim Eun-Joo
Kim Soo-Kyung
Lee Jang-Cheon
Lim Kyu-Sang
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Abstract
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Objective : The goal of this study is to identify the effects of extract ofGentianae sino-ornata(GSO) on the anti-oxidative activity of skin.For this purpose, several functions of GSO were analyzed in terms of skin-lightening activity and wrinkle improvement.
Methods : Cell viability was measured by neutral red (NR) assay, and GSO showed highly efficacy in DPPH radical scavenging activity. The level of tyrosinase and matrix metalloproteinase-1 (MMP-1) in media was analyzed by ELISA kit, and the expressions of p-JNK and p-ERK was measured by Western blot. To elucidate inhibitory effects of GSO on melanin synthesis, I determined the tyrosinase activity and melanin production in B16F10 cells.
Results : MMP-1 production in UVB-stimulated HDF cells was inhibited by GSO treatments, and also GSO inhibited protein expression levels of p-JNK and p-ERK. GSO significantly reduced tyrosinase activity and melanin synthesis in B16F10 cells.
Conclusions : From these results, GSO appears to be effective on skin elasticity increase, wrinkle improvement, whitening as anti-aging activity.
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KEYWORD
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ERK, JNK, Gentuanae sino-ornata, MMP-1, melanin, tyrosinase, α-MSH
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